OLEG ALFEROV, PH.D. Argonne National Laboratory 310 Woodcreek Dr., apt. #209 9700 South Cass Avenue Bolingbrook, IL 60440 Argonne, IL 60439 Phone: (630) 460-0267 Phone: (630) 252-3902 E-mail: oalferov@hyenapaws.com Fax: (630) 252-9155 E-mail: oalferov@anl.gov SUMMARY ---------- Research goals * Development of automated algorithms for statistical analysis of experi mental data, pattern recognition, and computer modeling of complex systems. * Hardware development of scientific or experimental devices, with emphasis on multi-functional, portable and embedded systems. Research interests * Algorithms for high-speed image processing, spot finding and data retrieval. * Algorithms for bacteria recognition with high reliability and low false-positives. * Automatic, user-friendly software for controlling scientific experiments, and for data processing and interpretation, including thermal dissociation process. * Development of custom research hardware, computer drivers, front-ends and controls. Research skills * Applied mathematics: statistical models, numerical methods for solving differential equations, combinatorial analysis, Fourier transform, curve fitting for custom process models, pattern recognition. * Bioinformatics: alignments, phylogenetic trees, probe selection. * Fluorescent microscopy, geometry optics. * Electronics design, including microcontroller-based systems. * Programming languages and software development: C/C++, LabVIEW, Python, Basic/Visual Basic, PHP, Pascal/Delphi, FORTRAN, JavaScript, TeX. * Computer platforms: Windows 95/98/NT/2000, Linux, DOS, Apple Macintosh, MSX-1/2, PDP/RSX; * Microcontroller platforms: Zilog eZ80, Rabbit2000, Basic Stamp, Motorola MC68 family. * Database tools: MS Access, MySQL, ODBC interface. PROFESSIONAL EXPERIENCE -------------------------- ARGONNE NATIONAL LABORATORY, Argonne, Illinois, USA Biochip Technology Center, Energy Systems Division Assistant Mathematician 1999 - Present * Developed automated statistical algorithms for high-speed pathogen recognition with high reliability of interpretation and low probability of false-positive calls using hybridization image acquired by CCD camera. * Developed fully automated laboratory fluorescent scanning microscope with uniform illumination, multicolor capability, wide-range temperature stabilization, fast temperature response, and humidity control. This device is used in the Biochip Center at Argonne as a tool for biological experiments with hybridization microarrays, including thermal dissociation experiments. * Developed control software for the microscopic equipment in the Biochip Center at Argonne, to conduct experiments, acquire and analyze images, and retrieve numerical data. The project included creation of custom drivers for various CCD cameras and other hardware controllers. * Developed tool for analysis of thermal dissociation curves that calculates the transition temperature and the speed of transition, which is immune to experimental noise. * Participated in development of the portable version of the thermally controlled laser-powered multicolor microscope. * Participated in development of drivers and control software for custom microarray manufacturing robots. * Participated in development of databases for biochip manufacture and quality control. * Administrated local file server for group of 15 researchers for data access and sharing in heterogeneous environment. RUSSIAN ACADEMY OF SCIENCES, Moscow, Russia Institute for Problems in Mechanics Research Associate 1995 - 1999 * Developed differential numerical scheme for calculation of turbulent flow with presence of Coriolis force and small obstacles on the surface. * Created a tool for web-based data upload and retrieval with configurable permissions. * Developed microcontroller-based pocket Morse code reader and transmitter with automatic recognition, adjustment of reception, and logging capability via serial port. MOSCOW STATE UNIVERSITY, Moscow, Russia Student 1990 - 1995 * Studied turbulent flow of liquid over a rotating rough surface and numerical and analytical methods for solving differential equations. EDUCATION ------------ RUSSIAN ACADEMY OF SCIENCES, Moscow, Russia. Institute for Problems in Mechanics Ph.D., Physics and Mathematics 1999 Supervisor: Dr. Alexander G. Petrov MOSCOW STATE UNIVERSITY, Moscow, Russia. Faculty of Mechanics and Mathematics M.S., Mechanics, Applied Mathematics 1995 PATENTS ---------- * U.S. Patent #6,620,623 Gennady Yershov, Oleg Alferov, Alexander Kukhtin Biochip Reader with Enhanced Illumination and Bioarray Positioning Apparatus Filed: May 6, 2001. SOFTWARE COPYRIGHTS ---------------------- * MicroChip Imager, Argonne National Laboratory, 2002. * MicroChip Reader, Argonne National Laboratory, 2002. * Universal Positioner, Argonne National Laboratory, 2004. HONORS AND AWARDS -------------------- Argonne National Laboratory Pacesetter Award, 2001. PROFESSIONAL SOCIETIES ------------------------- American Association for the Advancement of Science. CITIZENSHIP -------------- Russian citizen. Applied for Green Card, receiving work permit in June 2005. PUBLICATIONS AND PRESENTATIONS --------------------------------- 1. Bavykin S., Lysov Y., Zakhariev V., Kelly J., Alferov O., Kukhtin A., Ershov G., Chernov B., Golova J., Jackman J., Stahl D., Cherni A., Chandler D. rRNA and gyrB Genes as a New Tool for Identification of Bacillus cereus Group Microorganisms. 4th SISPAT Singapore International Symposium on Protection Against Toxic Substances, Singapore, 2004. 2. Yershov G., Kukhtin A., Chernov B., Golova J., Alferov O., Nelson M., Chandler D. Characterization and Standardization of "Biochip-Sample-Reader" System. IBC's 11th Annual International Microarray and Microtechnology Conference (Chips to Hits), Boston, USA, 2004. 3. Yershov G., Alferov O., Kukhtin A., Pates D., Perov A., Nelson M., Chernov B., Golova J., Pedrac C. Development of Multicolor Reader With Thermocontrolled Biochip Holder And Flow Cell Option. Argonne National Laboratory, Argonne, USA, 2004. 4. Yershov G., Alferov O., Kukhtin A. Instrument and Software for Bacteria Identification on 3D Biochip Platform. Biological Agent Identification Technologies (BAIT 2002, DARPA), LasVegas, USA, 2002. 5. Kukhtin A., Alferov O., Yershov G., Bavykin S., Hamman K., Regunathan A., Bronuskas J. Detection Limits for Bacteria Identification and Reusability for ANL 3D Biochip Platform. Biological Agent Identification Technologies (BAIT 2002, DARPA), LasVegas, USA, 2002. 6. Jackman J., Bavykin S., Sullivan P., Kukhtin A., Yershov G., Alferov O., Kelly J., Stahl D. Rapid species-specific identification of bacteria without PCR using microarray technology. Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Defense and Law Enforcement. Proc. of SPIE, Vol. 4708. AeroSense, 2002. 7. Jackman J., Bavykin S., Sullivan P., Barsky V., Kukhtin A., Yershov G., Alferov O., Kelly J., Stahl D., Mirzabekov A. Rapid Species-Specific Identification Of Bacteria Without PCR. Seventh Int. Symposium on Protection Against Chemical and Biological Warfare Agents, Stockholm, Sweeden, 2001. 8. Alferov O.S., Nekrasov I.V., Petrov A.G. Turbulent Flow over a Gently Sloping Two-Dimensional Hill. Izvestia, Atmospheric and Oceanic Physics, Vol. 36, No. 1, 2000, pp. 48-56. 9. Alferov O.S., Petrov A.G. Two-Layer Turbulent Flow Over a Rough Rotating Surface. Fluid Dynamics, Vol. 30, No. 4, 1995, pp. 537-543. 10. Alferov O.S., Petrov A.G. Turbulent Flow Over Rotating Rough Surface. Proc. of the Second Int. Conf. AiM'96, Saint Petersburg, Russia, 1997, pp.1-8. PERSONAL INTERESTS --------------------- Radio ham call sign: KC9GFV, issued in June 2004. Member of furry fandom since 2001. REFERENCES ------------- Available upon request.